LOCUS       V006298                 7733 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006298
VERSION     V006298
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7733)
  AUTHORS   Ding K, Zhang X, Mrazek J, Kickhoefer VA, Lai M, Ng HL, Yang OO,
            Rome LH, Zhou ZH.
  TITLE     Solution Structures of Engineered Vault Particles
  JOURNAL   Structure (2018) In press
   PUBMED   29551289
REFERENCE   2  (bases 1 to 7733)
  AUTHORS   Ding K, Zhang X, Mrazek J, Kickhoefer VA, Lai M, Ng HL, Yang OO,
            Rome LH, Zhou H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2018) Bioengineering, University of California,
            Los Angeles, 405 Hilgard Ave, Los Angeles, CA 90034, USA
REFERENCE   3  (bases 1 to 7733)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7733)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Structure
            (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-MAR-2018) Bioengineering, University of California, Los Angeles,
            405 Hilgard Ave, Los Angeles, CA 90034, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..457
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        484..588
                     /label="AmpR promoter"
     CDS             589..1446
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1620..2208
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     mobile_element  complement(2511..2735)
                     /label="Tn7R"
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2805..3335)
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     promoter        complement(3524..3552)
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     promoter        3904..3995
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             4287..6869
                     /gene="Mvp"
                     /label="Major vault protein"
                     /note="Major vault protein from Rattus norvegicus.
                     Accession#: Q62667"
     polyA_signal    7223..7357
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     mobile_element  complement(7386..7551)
                     /label="Tn7L"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
     2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
     3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
     3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
     3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
     3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
     3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
     4021 ccatcgggcg cggatccttg tttggtggga aaagcttggg attcaccatg accccccgga
     4081 ccctgaacgc ctgggtcaag gtggtggaag agaaggcctt cagccccgaa gtgatcccca
     4141 tgttcaccgc cctgagcgag ggcgccaccc ccagcgacct gaacaccatg ctgaatacca
     4201 tcggcggcca ccaggccgcc atgcagatgc tgaaggacac catcaacgaa gaggccgccg
     4261 agtgggaccg gggtttcctg ggtctcatgg caactgaaga ggccatcatc cgcatccccc
     4321 cataccacta catccatgtg ctggaccaga acagtaatgt gtcccgtgtg gaggttggac
     4381 caaagaccta catccggcag gacaatgaga gggtactgtt tgccccagtt cgcatggtga
     4441 ccgtcccccc acgccactac tgcatagtgg ccaaccctgt gtcccgggac acccagagtt
     4501 ctgtgttatt tgacatcaca ggacaagtcc gactccggca cgctgaccag gagatccgac
     4561 tagcccagga ccccttcccc ctgtatccag gggaggtgct ggaaaaggac atcaccccac
     4621 tgcaggtggt tctgcccaac acagcactgc atcttaaggc gttgctggac tttgaggata
     4681 agaatggaga caaggtcatg gcaggagacg agtggctatt tgagggacct ggcacctaca
     4741 tcccacagaa ggaagtggaa gtcgtggaga tcattcaggc cacagtcatc aaacagaacc
     4801 aagcactgcg gctaagggcc cgaaaggagt gctttgaccg ggagggcaag gggcgcgtga
     4861 caggtgagga gtggctggtc cgatccgtgg gggcttacct cccagctgtc tttgaagagg
     4921 tgctggatct ggtggatgct gtgatcctta cagaaaagac tgccctgcac ctccgggctc
     4981 tgcagaactt cagggacctt cggggagtgc tccaccgcac cggggaggaa tggttagtga
     5041 cagtgcagga cacagaagcc catgttccag atgtctatga ggaggtgctt ggggtagtac
     5101 ccatcaccac cctgggacct cgacactact gtgtcattct tgacccaatg ggaccagacg
     5161 gcaagaacca gctgggacaa aagcgtgttg tcaagggaga gaagtccttt ttcctccagc
     5221 caggagagag gctggagcga ggcatccagg atgtgtatgt gctgtcagag cagcaggggc
     5281 tgctactgaa ggcactgcag cccctggagg agggagagag cgaggagaag gtctcccatc
     5341 aggccggaga ctgctggctc atccgtgggc ccctggagta tgtgccatct gcaaaagtgg
     5401 aggtggtgga ggagcgtcag gctatccctc tggaccaaaa tgagggcatc tatgtgcagg
     5461 atgtcaagac ggggaaggtg cgggctgtga ttggaagcac ctacatgctg actcaggatg
     5521 aagtcctgtg ggaaaaggag ctgccttctg gggtggagga gctgctgaac ttggggcatg
     5581 accctctggc agacaggggt cagaagggca cagccaagcc ccttcagccc tcagctccaa
     5641 ggaacaagac ccgagtggtc agctaccgtg tcccgcacaa tgcagcggtg caggtctatg
     5701 actacagagc caagagagcc cgtgtggtct ttgggcccga gctagtgaca ctggatcctg
     5761 aggagcagtt cacagtattg tccctttctg ccgggcgacc caagcgtcct catgcccgcc
     5821 gtgcactctg cctactgctg ggacctgatt tctttactga tgtcatcacc atcgaaactg
     5881 cagatcatgc caggttgcag ctgcagcttg cctacaactg gcactttgaa ctgaagaacc
     5941 ggaatgaccc tgcagaggca gccaagcttt tctccgtgcc tgacttcgtg ggtgacgcct
     6001 gcaaggccat tgcatcccga gtccgggggg ctgtagcctc tgtcaccttt gatgacttcc
     6061 ataaaaactc agcccggatc attcgaatgg ctgtttttgg ctttgagatg tctgaagaca
     6121 caggtcctga tggcacactc ctgcccaagg ctcgagacca ggcagtcttt ccccaaaacg
     6181 ggctggtagt cagcagtgtg gatgtgcagt cagtggagcc cgtggaccag aggacccggg
     6241 atgcccttca gcgcagcgtt cagctggcca tcgaaattac caccaactcc caggaggcag
     6301 cagccaagca cgaggctcag agactggaac aggaagcccg tggtcggctt gagaggcaga
     6361 agatcttgga ccagtcagaa gctgaaaaag cccgcaagga actcttggag cttgaggcta
     6421 tgagcatggc tgtggagagc acgggtaatg ccaaagcaga ggctgagtcc cgtgcagagg
     6481 cagcgaggat cgaaggagaa ggctctgtgc tgcaggccaa gctcaaggca caggcgctag
     6541 ccattgagac ggaggctgag ttggagcgag taaagaaagt acgagagatg gaactgatct
     6601 atgcccgggc ccagttggag ctggaggtga gcaaggcgca gcagcttgcc aatgtggagg
     6661 caaagaagtt caaggagatg acagaggcac tgggccccgg caccatcagg gacctggctg
     6721 tggccgggcc agagatgcag gtgaaacttc tccagtccct gggcctgaaa tccactctca
     6781 tcaccgatgg ctcgtctccc atcaacctct tcagcacagc cttcgggttg ctggggctgg
     6841 ggtctgatgg tcagccgcca gcacagaagt gatccggcag cccggggaag acttgctctc
     6901 ccaggctctc cgaagcagcc atgctgtgcc ttaggtcaac actgactgca ctgacaatgg
     6961 ataaaataaa ttgacaactg taaaaaaaaa aaaaaagtcg acgcggccgc gaattcaaag
     7021 gcctacgtcg acgagctcac tagtcgcggc cgctttcgaa tctagagcct gcagtctcga
     7081 ggcatgcggt accaagcttg tcgagaagta ctagaggatc ataatcagcc ataccacatt
     7141 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     7201 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     7261 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7321 gtccaaactc atcaatgtat cttatcatgt ctggatctga tcactgcttg agcctaggag
     7381 atccgaacca gataagtgaa atctagttcc aaactatttt gtcattttta attttcgtat
     7441 tagcttacga cgctacaccc agttcccatc tattttgtca ctcttcccta aataatcctt
     7501 aaaaactcca tttccacccc tcccagttcc caactatttt gtccgcccac agcggggcat
     7561 ttttcttcct gttatgtttt taatcaaaca tcctgccaac tccatgtgac aaaccgtcat
     7621 cttcggctac tttttctctg tcacagaatg aaaatttttc tgtcatctct tcgttattaa
     7681 tgtttgtaat tgactgaata tcaacgctta tttgcagcct gaatggcgaa tgg
//
